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Chapters
- 00:05Title
- 01:28Generating Substrate: Polydimethylsiloxane (PDMS) Slides
- 02:49Generating Substrate: Polyacrylamide (PA) Slide
- 05:57Culturing Mammalian Cells on MEArrays for Functional Analysis
- 09:05Using MEArrays to Analyze How Type I Collagen and P-cadherin Affects Keratin Expression in Multipotent Mammary Epithelial Progenitor Cells
- 11:05Conclusion
A combinatorial functional screening method for gaining insights into the impacts of the molecular composition of microenvironments on cellular functions is described. The method takes advantage of existing microarray-based technologies to generate arrays of defined combinatorial microenvironments that support cell adhesion and functional analysis.
Tags
FabricationUseMicroEnvironment MicroArraysMEArraysInteractionsCellsMicroenvironmentFunctional ConsequencesSingle Cell LevelDifferentiationMigrationProliferation PhenotypesMorphogenesisTumorigenesisCell-cell InteractionsECM InteractionsSoluble Factor InteractionsPhysical CharacteristicsGenomic ContentCombinatorial InteractionsMicroenvironmental ComponentsDistinctive BehaviorsMEArray PlatformCell-based Functional ScreeningMolecular CompositionElastic ModulusMicroarray TechnologyMicropatterning Technology
