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制造和使用微环境芯片(MEArrays)
JoVE Journal
Biology
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JoVE Journal Biology
Fabrication and Use of MicroEnvironment microArrays (MEArrays)
DOI:

11:57 min

October 11, 2012

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Chapters

  • 00:05Title
  • 01:28Generating Substrate: Polydimethylsiloxane (PDMS) Slides
  • 02:49Generating Substrate: Polyacrylamide (PA) Slide
  • 05:57Culturing Mammalian Cells on MEArrays for Functional Analysis
  • 09:05Using MEArrays to Analyze How Type I Collagen and P-cadherin Affects Keratin Expression in Multipotent Mammary Epithelial Progenitor Cells
  • 11:05Conclusion

Summary

Automatic Translation

一个组合功能性筛选方法获得洞察的分子组成的微环境对细胞功能的影响。该方法充分利用了现有的基于微阵列技术来生成数组的定义组合的微环境,支持细胞的粘附和功能分析。

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