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制造和使用微环境芯片(MEArrays)
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Biology
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JoVE Journal
Biology
Fabrication and Use of MicroEnvironment microArrays (MEArrays)
Please note that all translations are automatically generated.
Click here for the English version.
制造和使用微环境芯片(MEArrays)
DOI:
10.3791/4152-v
•
11:57 min
•
October 11, 2012
•
Chun-Han Lin
2
,
Jonathan K. Lee
,
Mark A. LaBarge
1
Life Science Division
,
Lawrence Berkeley National Laboratory
,
2
Department of Comparative Biochemistry
,
University of California, Berkeley
Chapters
00:05
Title
01:28
Generating Substrate: Polydimethylsiloxane (PDMS) Slides
02:49
Generating Substrate: Polyacrylamide (PA) Slide
05:57
Culturing Mammalian Cells on MEArrays for Functional Analysis
09:05
Using MEArrays to Analyze How Type I Collagen and P-cadherin Affects Keratin Expression in Multipotent Mammary Epithelial Progenitor Cells
11:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个组合功能性筛选方法获得洞察的分子组成的微环境对细胞功能的影响。该方法充分利用了现有的基于微阵列技术来生成数组的定义组合的微环境,支持细胞的粘附和功能分析。
Tags
Fabrication
Use
MicroEnvironment MicroArrays
MEArrays
Interactions
Cells
Microenvironment
Functional Consequences
Single Cell Level
Differentiation
Migration
Proliferation Phenotypes
Morphogenesis
Tumorigenesis
Cell-cell Interactions
ECM Interactions
Soluble Factor Interactions
Physical Characteristics
Genomic Content
Combinatorial Interactions
Microenvironmental Components
Distinctive Behaviors
MEArray Platform
Cell-based Functional Screening
Molecular Composition
Elastic Modulus
Microarray Technology
Micropatterning Technology
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