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Chapters
- 00:05Title
- 03:04Substrate Preparation and Cell Culture
- 04:30Fabrication of PDMS VMMC
- 06:37Cell Shearing
- 08:30Monocyte Adhesion Assay
- 10:55Representative Results
- 11:27Conclusion
Câmaras de fluxo microfluídicos gravados por fotolitografia e fabricada a partir de PDMS são aplicados para sondar os resultados funcionais associados com disfunção CE e inflamação. Numa experiência representativa, a capacidade de tensão de cisalhamento diferencial para modular a adesão celular monocítica a citocina activado CE monocamadas é demonstrada.
Tags
On-Chip Endothelial Inflammatory PhenotypingAtherogenesisMetabolic AbnormalitiesSystemic InflammationEndothelial DysfunctionFunctional Changes In EndotheliumRisk AssessmentTherapeutic StrategyRegulation Of InflammationLocal HemodynamicsNon-random Spatial Distribution Of AtherosclerosisLab-on-a-chipQuantitatively Assay Metabolic PerturbationInflammatory Events In Endothelial CellsMonocytesFlow ConditionsSoft LithographyMicrofabricate Vascular Mimetic Microfluidic Chambers (VMMC)Cultured EC MonolayersImaging Inflammatory EventsShear FieldCytokine-induced InflammationLipid-induced InflammationRAGE-induced InflammationHuman Aortic EC (HAEC)Monocytic Cell Rolling And ArrestTNF-alpha
