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免电力，顺序的核酸和蛋白质的分离
JoVE Journal
Biology
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JoVE Journal Biology
Electricity-Free, Sequential Nucleic Acid and Protein Isolation
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免电力，顺序的核酸和蛋白质的分离

DOI:
10.3791/4202-v

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09:52 min

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May 15, 2012

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,
1CUBRC, Inc., 2Department of Microbiology and Immunology,State University of New York at Buffalo, School of Medicine and Biomedical Sciences

Chapters

  • 00:05Title
  • 02:20Sample Lysis
  • 03:20Nuclei Acid Extraction
  • 05:27Protein Extraction
  • 07:11Results: Sequential Nucleic Acid and Protein Isolation
  • 09:28Conclusion

Summary

Automatic Translation

Automatic Translation

工具和化学描述顺序隔离没有对电力的需要，由蛋白质样品的核酸。该工具由吸附剂而隔离化学固相萃取原则的基础上，移液管内举行。孤立的大分子可以基于免疫和基于PCR的检测分析。

Tags

Electricity-freeSequentialNucleic Acid IsolationProtein IsolationTraditional PathogensEmerging PathogensEnterohemorrhagic Escherichia Coli (EHEC)Yersinia PestisPrion-based DiseasesConcernGovernmentsIndustriesMedical ProfessionalsDeathsChildrenDeveloping WorldLaboratory-based IdentificationUnited StatesLow CostField-based ToolsPoint-of-careRapid IdentificationDiagnosisDisease MarkersDisease ProgressionPatient PrognosisSolid-phase Extraction (SPE)Nucleic AcidsProteinsSample Isolation ToolBOOM TechnologyChaotropic Salt Solution

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