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Immunology and Infection
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区分功能基因表达的作用,通过骨髓移植免疫和非免疫细胞在小鼠结肠炎
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
Please note that all translations are automatically generated.
Click here for the English version.
区分功能基因表达的作用,通过骨髓移植免疫和非免疫细胞在小鼠结肠炎
DOI:
10.3791/4208-v
•
14:43 min
•
October 01, 2012
•
Hon Wai Koon
,
Samantha Ho
,
Michelle Cheng
,
Ryan Ichikawa
,
Charalabos Pothoulakis
1
Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine
,
The University of California Los Angeles, Los Angeles
Chapters
00:05
Title
01:26
Experimental Setup
02:39
Donor Bone Marrow Extraction
05:49
Infusion to Recipient Mice
07:19
Induction of Colitis and Evaluation of Colitis
08:55
Quality Inspection of Bone Marrow Transplantation by Flow Cytometry
11:59
Transplantation Results
14:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
骨髓移植提供了一种方法来改变的骨髓衍生细胞的基因型。如果感兴趣的基因的表达在两个骨髓来源的细胞和非骨髓来源的细胞,骨髓移植可以改变到一个不同的基因型的骨髓来源的细胞,不改变非骨髓来源的细胞的基因型。
Tags
Gene Expression
Immune Cells
Non-immune Cells
Mouse Colitis
Bone Marrow Transplantation
Gene-of-interest
Knockout Mice
Genotype
Irradiation
Donor Bone Marrow
Recipient Mice
Wild-type Mice
CD Molecules (CD45.1 And CD45.2)
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