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Differenziert Functional Roles of Gene Expression von Immun-und Nicht-Immunzellen in Mouse Colitis von Bone Marrow Transplantation
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
DOI:

14:43 min

October 01, 2012

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Chapters

  • 00:05Title
  • 01:26Experimental Setup
  • 02:39Donor Bone Marrow Extraction
  • 05:49Infusion to Recipient Mice
  • 07:19Induction of Colitis and Evaluation of Colitis
  • 08:55Quality Inspection of Bone Marrow Transplantation by Flow Cytometry
  • 11:59Transplantation Results
  • 14:21Conclusion

Summary

Automatic Translation

Knochenmarktransplantation bietet eine Möglichkeit, um den Genotyp der Knochenmark stammende Zellen zu ändern. Wenn das Gen von Interesse an beiden Knochenmark stammende Zellen und nicht-Knochenmark stammende Zellen exprimiert wird, kann die Knochenmarktransplantation Knochenmark stammende Zellen zu einem anderen Genotyp ändern, ohne die nicht-Knochenmark stammende Zellen Genotyp.

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