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在GFP标记的下丘脑神经元的小鼠脑片成像钙反应
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
Please note that all translations are automatically generated.
Click here for the English version.
在GFP标记的下丘脑神经元的小鼠脑片成像钙反应
DOI:
10.3791/4213-v
•
09:14 min
•
August 24, 2012
•
Christian Schauer
,
Trese Leinders-Zufall
1
Department of Physiology, School of Medicine
,
University of Saarland, Homburg, Germany
Chapters
00:05
Title
01:52
Solution Preparation and Brain Dissection
03:21
Coronal Hypothalamic Sections Slicing
04:30
Preparation of the Ca
2+
Indicator Dye Loading Solution
06:35
Microscopy and Analysis
07:59
Results: Ca
2+
Signals in
T
GFP Neurons of Mouse Hypothalamic Brain Slices
08:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个协议中,我们更新了最新进展,成像钙<sup> 2 +</sup>绿色荧光蛋白标记的神经元在脑组织切片,使用红色荧光钙信号<sup> 2 +</sup>指示剂染料。
Tags
Imaging
Calcium Responses
GFP-tagged Neurons
Hypothalamic Mouse Brain Slices
Encoding Of Information
Molecular Steps
Neurohormone Signal Transduction Cascades
Intracellular Ca2+
Synaptic Sites
Green Fluorescent Protein (GFP)
Spatially And Temporally Stimulus-induced Ca2+ Responses
Non-green Fluorescent Ca2+ Indicator Dye
Confocal Microscopy
Ratiometric Ca2+ Indicator Fura-2
Ultraviolet (UV) Light
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