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在GFP标记的下丘脑神经元的小鼠脑片成像钙反应
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
DOI:

09:14 min

August 24, 2012

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Chapters

  • 00:05Title
  • 01:52Solution Preparation and Brain Dissection
  • 03:21Coronal Hypothalamic Sections Slicing
  • 04:30Preparation of the Ca2+ Indicator Dye Loading Solution
  • 06:35Microscopy and Analysis
  • 07:59Results: Ca2+ Signals in TGFP Neurons of Mouse Hypothalamic Brain Slices
  • 08:52Conclusion

Summary

Automatic Translation

在这个协议中,我们更新了最新进展,成像钙<sup> 2 +</sup>绿色荧光蛋白标记的神经元在脑组织切片,使用红色荧光钙信号<sup> 2 +</sup>指示剂染料。

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