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Journal
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Immunology and Infection
/
piggyBac转座子
初级人类T细胞系统改造
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
piggyBac
Transposon System Modification of Primary Human T Cells
Please note that all translations are automatically generated.
Click here for the English version.
piggyBac转座子
初级人类T细胞系统改造
DOI:
10.3791/4235-v
•
10:02 min
•
November 05, 2012
•
Sunandan Saha
2
,
Yozo Nakazawa
,
Leslie E. Huye
5
,
Joseph E. Doherty
6
,
Daniel L. Galvan
,
Cliona M. Rooney
5,7
,
Matthew H. Wilson
2,4,8
1
Program in Translational Biology and Molecular Medicine
,
Baylor College of Medicine
,
2
Department of Medicine, Division of Nephrology
,
Baylor College of Medicine
,
3
Department of Immunology and Pathology
,
Shinshu University School of Medicine
,
4
Center for Cell and Gene Therapy
,
Baylor College of Medicine
,
5
Department of Pediatrics
,
Baylor College of Medicine
,
6
Program in Cell and Molecular Biology
,
Baylor College of Medicine
,
7
Department of Molecular Virology and Microbiology
,
Baylor College of Medicine
,
8
Michael E. DeBakey VA Medical Center
Chapters
00:05
Title
01:39
Day 0: Isolation of PBMCs from Human Blood
03:23
Day 1: Coating Plates with anti-CD28 and anti-CD3 Antibody for Stimulating T Cells
03:59
Day 1: Nucleofection of Unstimulated T Cells
05:54
Day 2: Nonspecific Stimulation of T Cells
07:28
Day 7: Analysis of Gene Expression
08:05
Results: piggyBac-mediated Gene Modification of Human T Cells
09:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了一种方法,初级人类T细胞的转基因使用的非病毒基因修饰<em>座子</em>转座子系统。修改,以使用的T细胞<em>座子</em转座系统具有稳定的转基因表达。
Tags
PiggyBac Transposon System
Primary Human T Cells
Gene Transfer
Plasmid
Transposase Enzyme
Inverted Repeat Elements
Gene Delivery
Adoptive Immunotherapy
Cancer
Clinical Trial
Non-viral Methodology
Genetic Modification
Reporter Gene
Inducible Suicide Gene
Genomic Integration Sites
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