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Hurtig Isolering af levedygtige cirkulerende tumorceller fra Patient Blodprøver
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
DOI:

07:32 min

June 15, 2012

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Chapters

  • 00:05Title
  • 01:15Preparation of Halloysite Nanotube Solution
  • 01:48Coating of Microtube Inner Surface with Halloysite Nanotubes
  • 03:10Preparation of Microtubes for Cell Isolation
  • 04:05Preparation of Samples for Cell Isolation
  • 05:07Cell Isolation
  • 06:18Results: Isolation of Circulating Tumor Cells (CTCs)
  • 07:10Conclusion

Summary

Automatic Translation

Cirkulerende tumorceller isoleres fra blodet hos cancerpatienter uden at påføre celleskade. Isolering af tumorceller opnås ved anvendelse af en bimolekylær overflade af E-selectin i tillæg til antistoffer mod epitel markører. En nanorør coating specifikt fremmer cancer celleadhæsion resulterer i høje capture renheder.

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