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3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting
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Bioengineering
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JoVE Journal Bioengineering
3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

DOI:
10.3791/62252-v

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05:32 min

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April 21, 2021

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, , , , , ,
1Biomanufacturing Center, Dept. of Mechanical Engineering,Tsinghua University, 2Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, 3Department of Mechanical Engineering,Drexel University

Chapters

  • 00:05Introduction
  • 00:56Nozzles and Hydrogel Bioink Preparation
  • 01:39Microdroplet Formation
  • 02:20Microcarrier Formation
  • 02:52Microcarrier Inoculation and Analysis
  • 03:52Results: 3D-Printed In Vitro Hydrogel Microcarrier Analysis
  • 04:48Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a mild 3D printing technique driven by alternating viscous-inertial forces to enable the construction of hydrogel microcarriers. Homemade nozzles offer flexibility, allowing easy replacement for different materials and diameters. Cell binding microcarriers with a diameter of 50-500 µm can be obtained and collected for further culturing.

Tags

3D PrintingHydrogel MicrocarriersAlternating Viscous-inertial Force JettingBioprintingIn Vitro Cell ExpansionMicrocarrier PreparationSodium AlginateCrosslinkingCell EncapsulationA549 CellsCell Tracker Dye

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