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Immunology and Infection

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枚举主要外周血白细胞人口多中心临床试验，使用全血表型分析

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Immunology and Infection
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JoVE Journal Immunology and Infection

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Please note that all translations are automatically generated. Click here for the English version.

枚举主要外周血白细胞人口多中心临床试验，使用全血表型分析

DOI:

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14:45 min

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September 16, 2012

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Tiffany R. Hensley, Austin B. Easter, Sarah E. Gerdts, Stephen C. De Rosa, Antje Heit, M. Juliana McElrath, Erica Andersen-Nissen

¹Vaccine and Infectious Disease Division,Fred Hutchinson Cancer Research Center

Chapters

00:00Title
00:10Background and Significance
02:08Reagents
03:37Preparation
05:04Staining
07:54Shipping
09:46Analysis
10:51Representative Data
13:42Conclusion

Summary

Automatic Translation

在这份报告中，我们展示了染色和分析步骤，新鲜全血进行列举主要的先天免疫和适应性白细胞种群的表型分析。执行这些程序在多中心临床试验的背景下，我们强调的注意事项。

Tags

Peripheral Blood Leukocyte Populations Multicenter Clinical Trials Whole Blood Phenotyping Assay Cryopreservation Immune Response Evaluations Granulocytes B Cells Dendritic Cells Fresh Leukocytes Assay Volunteer Commitments Timeframes Working Hours Laboratory Personnel Multiple Centers 11-color Antibody Staining Panel Trucount Tubes Phenotype Enumeration

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