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Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas
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Chapters
- 00:05Title
- 01:26Dissection of Larval Eye Imaginal Discs
- 04:33Dissection of 50% Pupation Retinas
- 07:42Dissection of Adult Eyes
- 10:19Antibody Stained Retinas from Three Developmental Stages
- 11:28Conclusion
The Drosophila retina is a crystal-like lattice composed of a small number of cell types that are generated in a stereotyped manner 1. Its amenability to sophisticated genetic analysis allows the study of complex developmental programs. This protocol describes dissections and immunohistochemistry of retinas at three discrete developmental stages, with a focus on photoreceptor differentiation.
Tags
DissectionImmunohistochemistryLarval Drosophila RetinasPupal Drosophila RetinasAdult Drosophila RetinasCompound EyeOmmatidiaCellsCone CellsPigment CellsBristle CellPhotoreceptors (PRs)RhabdomeresRhodopsins (Rhs)Rh1R1-R6 PRsR7 PRsR8 PRsSubtypesLarval Eye-antennal Imaginal DiscEpithelial CellsDifferentiation WaveOmmatidia AssemblyFounder Cell R8Pupal DevelopmentMorphological ChangesRhabdomere FormationSynaptogenesisRh Expression
