Neuroscience
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カーボンブラックインクの血管内灌流は脳血管の信頼性の高い可視化を可能に
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Summary January 4th, 2013
Please note that all translations are automatically generated.
Click here for the English version.
げっ歯類の脳の解剖学的構造の分析は、実験的な脳卒中の研究に重要な役割を果たしている。この文脈において、着色ラテックスと血管内血流は数年のための標準ツールとして考えられてきた。しかし、この技術は、その再現性を弱体化させる明確な技術的な限界を意味しています。ここでは、再現可能な方法で脳の血管を可視化するための簡単な方法を説明します。高コントラストの可視化と脳血管の適切な充填における左心室の心筋の結果を介して2つの市販のカーボンブラックインクの混合物の注入。我々は、正常に異なる遺伝的背景をもつマウスの脳血管地域間吻合のポイントを識別するためにこの手法を適用している。マウスでの梗塞体積を観察し、分析するための広く使用されているツール - 我々は最終的に血管染色にこの小説と単純な方法は、塩化トリフェニルテトラゾリウム(TTC)染色と組み合わせることができるという証拠を与える。
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