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Barwy Pasażowanie ludzkich embrionalnych komórek macierzystych linii Trypsyna
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JoVE Journal Biology
Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

Barwy Pasażowanie ludzkich embrionalnych komórek macierzystych linii Trypsyna

DOI:
10.3791/49-v

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09:38 min

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October 12, 2006

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, ,
1Department of Molecular and Cell Biology,Harvard

Chapters

  • 00:06ES cells in culture
  • 00:28Preparation
  • 02:06Trypsinize cells
  • 04:46Separate ES cells and feeder cells
  • 06:59Resuspend and split cells

Summary

Automatic Translation

Automatic Translation

W tym filmie pokazujemy, jak w naszym laboratorium rutynowo fragmenty barwy linii ludzkich zarodkowych komórek macierzystych, z trypsyny.

Tags

BiologyTrypsinCell CultureKaryotypic AbnormalitiesUndifferentiatedCulture MaintenanceTrypsin-EDTACell SuspensionHuES MediaSplit RatioRe-platingMouse Embryonic Fibroblast Feeder CellsMedia Change

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