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Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

JoVE Journal
Biology

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JoVE Journal Biology

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

DOI:

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09:38 min

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October 12, 2006

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Erin Trish, John Dimos, Kevin Eggan

¹Department of Molecular and Cell Biology,Harvard

Chapters

00:06ES cells in culture
00:28Preparation
02:06Trypsinize cells
04:46Separate ES cells and feeder cells
06:59Resuspend and split cells

Summary

Automatic Translation

In this video we demonstrate how our lab routinely passages HuES human embryonic stem cell lines with trypsin. Brought to you by JoVE.

Tags

Biology Trypsin Cell Culture Karyotypic Abnormalities Undifferentiated Culture Maintenance Trypsin-EDTA Cell Suspension HuES Media Split Ratio Re-plating Mouse Embryonic Fibroblast Feeder Cells Media Change

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