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Passage af Hues humane embryonale stamceller-linjer med Trypsin

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Biology

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JoVE Journal Biology

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

Passage af Hues humane embryonale stamceller-linjer med Trypsin

DOI:

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09:38 min

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October 12, 2006

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Erin Trish, John Dimos, Kevin Eggan

¹Department of Molecular and Cell Biology,Harvard

Chapters

00:06ES cells in culture
00:28Preparation
02:06Trypsinize cells
04:46Separate ES cells and feeder cells
06:59Resuspend and split cells

Summary

Automatic Translation

I denne video viser vi, hvordan vores laboratorium rutinemæssigt passager farvetone humane embryonale stamcellelinjer med trypsin.

Tags

Biology Trypsin Cell Culture Karyotypic Abnormalities Undifferentiated Culture Maintenance Trypsin-EDTA Cell Suspension HuES Media Split Ratio Re-plating Mouse Embryonic Fibroblast Feeder Cells Media Change

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