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同时光感受器和二阶神经元在两栖类动物的视网膜切片制备全细胞记录

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation

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同时光感受器和二阶神经元在两栖类动物的视网膜切片制备全细胞记录

DOI:

10.3791/50007-v

•

11:39 min

•

June 01, 2013

•

Matthew J. Van Hook, Wallace B. Thoreson²

¹Department of Ophthalmology and Visual Sciences,University of Nebraska Medical Center , ²Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center

Chapters

00:05Title
01:57Retinal Slices Preparation
06:13Paired Whole-cell Recordings
09:21Results: Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
11:00Conclusion

Summary

Automatic Translation

我们描述了制备薄视网膜片从水产虎蝾螈（<em> Ambystoma tigrinum</em>），并解释我们如何使用这些切片研究获得双全细胞电压钳记录，从感光细胞和二阶的水平细胞和双极细胞在视网膜突触处理。

Tags

Keywords: Retinal Neuroscience Retinal Circuitry Photoreceptors Retinal Neurons Electrophysiological Recording Vertical Retinal Slice Dual Whole-cell Voltage Clamp Photoreceptor Membrane Potential Synaptic Ribbon Neurotransmitter Release

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