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I ovo Angivelse af MicroRNA i Ventral Chick Midbrain
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
In ovo Expression of MicroRNA in Ventral Chick Midbrain
DOI:

09:19 min

September 16, 2013

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Chapters

  • 00:00Title
  • 01:53Introduction
  • 02:23Finishing and filling of micropipettes
  • 03:00Incubation and preparation of eggs
  • 04:43DNA injection into midbrain/neural tube
  • 05:37Electroporation of midbrain
  • 07:06Removing, cleaning and fixing of embryo or fetus
  • 08:23Conclusion

Summary

Automatic Translation

Ektopisk udtryk er en teknik til at belyse microRNA rolle i hjernens udvikling. Men rettet mod specifikke områder ved hjælp af in ovo elektroporation er udfordrende. Her viser vi en effektiv måde til selektivt elektroporere ventrale og dorsale midthjernen regioner.

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