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JoVE Journal Neuroscience
Organotypic Slice Cultures of Embryonic Ventral Midbrain
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Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

Article DOI: 10.3791/3350 • 07:34 min • January 31st, 2012
January 31st, 2012 •
1, 1
1Institute of Reconstructive Neurobiology, University of Bonn

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A method to generate organotypic slices from the E12.5 murine embryonic midbrain is described. The organotypic slice cultures can be used to observe the behavior of dopaminergic neurons or other ventral midbrain neurons.

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Organotypic Slice Cultures Embryonic Ventral Midbrain Dopaminergic Neuronal Development In Vitro Mouse Model Organism Brain Development Molecular Data Genetic Data Organotypic Slice Cultures Of Embryonic Brains Murine Brain Development Ex-vivo Manipulation Transgenic Mice Gene Expression Fluorescent Proteins Time-lapse Imaging Cerebral Cortex Development Forebrain Ventral Brain Areas Neuronal Clusters Migratory Trajectories Final Position
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