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Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
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Neuroscience
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JoVE Journal Neuroscience
Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

DOI:
10.3791/3350-v

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07:33 min

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January 31, 2012

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,
1Institute of Reconstructive Neurobiology,University of Bonn

Chapters

  • 00:05Title
  • 01:00Preparation for Dissection
  • 03:00Embryonic Brain Preparation and Vibratome Sectioning
  • 05:09Slice Culture and Time-lapse Imaging
  • 06:21Results: Dopaminergic Neuronal Development in Organotypic Slice Cultures of Embryonic Ventral Midbrain
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

A method to generate organotypic slices from the E12.5 murine embryonic midbrain is described. The organotypic slice cultures can be used to observe the behavior of dopaminergic neurons or other ventral midbrain neurons.

Tags

Organotypic Slice CulturesEmbryonic Ventral MidbrainDopaminergic Neuronal DevelopmentIn VitroMouse Model OrganismBrain DevelopmentMolecular DataGenetic DataOrganotypic Slice Cultures Of Embryonic BrainsMurine Brain DevelopmentEx-vivo ManipulationTransgenic MiceGene ExpressionFluorescent ProteinsTime-lapse ImagingCerebral Cortex DevelopmentForebrainVentral Brain AreasNeuronal ClustersMigratory TrajectoriesFinal Position

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