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JoVE Journal
Immunology and Infection

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定量高通量单细胞的细胞毒性T细胞的测定
 
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定量高通量单细胞的细胞毒性T细胞的测定

Article DOI: 10.3791/50058
February 2nd, 2013

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Summary February 2nd, 2013

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我们描述了一种单细胞的高通量的检测,以测量与肿瘤靶细胞的培养时的细胞毒性T细胞。此方法采用致密的,弹性体子纳升井(〜100,000井/数组)的数组,在空间上局限在T细胞和靶细胞,在规定的比例,和被耦合到的荧光显微镜来监测效靶共轭和随后的细胞凋亡。

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