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Immunology and Infection
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用于嵌合抗原受体 T 细胞的实时效力测定,用于定位固体和血液癌细胞
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
用于嵌合抗原受体 T 细胞的实时效力测定,用于定位固体和血液癌细胞
DOI:
10.3791/59033-v
•
08:46 min
•
November 12, 2019
•
Biao Xi
,
Robert Berahovich
,
Hua Zhou
,
Shirley Xu
,
Yuehua Wei
,
Jasper Guan
,
Hizkia Harto
,
Jian Guan
,
Lijun Wu
,
David Santa Ana
3
,
Fabio Cerignoil
,
Brandon Lamarche
,
Yama A. Abassi
,
Vita Golubovskaya
1
ACEA Biosciences, Inc
,
2
ProMab Biotechnologies. Inc
,
3
ACEA Therapeutics
Chapters
00:04
Title
00:30
Chimeric Antigen Receptor (CAR)-Encoding Lentivirus Generation
02:29
CAR T Cell Generation and Expansion
03:16
CAR Expression Detection
04:55
Real-Time Cytolysis Potency Assay
06:48
Results: Representative CAR T Cell Target Cell Killing Efficacy as Assessed by RTCA
08:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个定量实时体外细胞解检测系统,以评估嵌合抗原受体T细胞针对液体和固体肿瘤细胞的效力。该协议可以扩展以评估其他免疫效应细胞,以及联合治疗。
Tags
Real-time Potency Assay
Chimeric Antigen Receptor T Cells
Solid And Hematological Cancer Cells
CELLigence Real-time Cell Analysis
Label-free Monitoring
Target Cancer Cell Killing
Effector Cell Types
Combination Therapies
Lentiviral Vector
HEK293FT Cells
Transfection
Lentivirus Production
Ultracentrifugation
Quantitative RT-PCR
CAR-T Cell Generation
Peripheral Blood Mononuclear Cells
CD3/CD28 Coated Microbeads
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