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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
T Hücreleri İçin Kantitatif Yüksek throughput Tek hücreli Sitotoksisite Testi
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
Journal (Immunology and Infection)
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
Journal (Immunology and Infection)
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
 
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T Hücreleri İçin Kantitatif Yüksek throughput Tek hücreli Sitotoksisite Testi

Article DOI: 10.3791/50058
February 2nd, 2013 •
1, 2, 1, 2, 1
1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center

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February 2nd, 2013

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Bu tümör hedef hücreleri ile inkübe zaman T hücrelerinin sitotoksisiteye ölçmek için bir tek hücreli yüksek verim tahlil açıklanmaktadır. Bu yöntem, mekansal olarak tanımlanan oranlarda T hücreler ve hedef hücrelerin sınırlandırmak için alt nanolitre kuyular (~ 100.000 kuyu / dizi) ve bir yoğun, elastomerik dizisi kullanır ve efektör-hedef konjugasyon ve daha sonra apoptoz izlemek için flüoresan mikroskop ile birleştirilmiştir.

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