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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantitativo de alta capacidade ensaio de citotoxicidade de célula única para as células T.
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Quantitativo de alta capacidade ensaio de citotoxicidade de célula única para as células T.

Article DOI: 10.3791/50058
February 2nd, 2013 •
1, 2, 1, 2, 1
1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center

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February 2nd, 2013

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Descrevemos uma única célula ensaio de elevado débito para medir a citotoxicidade de células T, quando incubado com as células-alvo tumorais. Este método emprega uma matriz elastomérica denso, de sub-nanolitro poços (~ 100000 poços matriz /) para confinar espacialmente as células T e as células alvo em proporções definidas e está acoplado a microscopia de fluorescência para monitorizar alvo efetuador conjugação e apoptose subsequente.

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