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目标移植的肌肉祖细胞的分子成像
JoVE Journal
Medicine
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JoVE Journal
Medicine
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
Please note that all translations are automatically generated.
Click here for the English version.
目标移植的肌肉祖细胞的分子成像
DOI:
10.3791/50119-v
•
09:24 min
•
March 27, 2013
•
Kelly Gutpell
2
,
Rebecca McGirr
,
Lisa Hoffman
2,3
1
Imaging Program
,
Lawson Health Research Institute
,
2
Department of Anatomy and Cell Biology
,
Western University
,
3
Department of Medical Biophysics
,
Western University
Chapters
00:05
Title
02:17
Culture and Transfection of C2C12 Myoblasts
04:26
Preparation of Myoblasts for Transplant
05:16
Cell Implantation
06:49
Injection of the Fluorescent Substrate
07:13
Bioluminescence Imaging to Image Luciferase-Expressing Myoblast Progenitor Cells
07:37
Results: Molecular Imaging to Target Transplanted Muscle Progenitor Cells
08:42
Conclusion
Summary
Automatic Translation
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非侵入性的方法来评估成肌细胞移植的成功。该方法利用一个统一的融合的记者基因组成的基因的表达,可以被不同的成像方式成像。在这里,我们使用<em>的波动</em>报告基因序列到靶细胞通过生物发光成像。
Tags
Molecular Imaging
Muscle Progenitor Cells
Duchenne Muscular Dystrophy
Bioluminescence Imaging
PET
SPECT
MRI
Ultrasound
Reporter Gene
C2C12 Myoblasts
Mdx Mouse
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