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Isolering og dyrkning af Neonatal Mouse cardiomyocytes
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Culture of Neonatal Mouse Cardiomyocytes
DOI:

07:50 min

September 06, 2013

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Chapters

  • 00:05Title
  • 01:33Day 1: Isolation of the Cardiac Tissue from Neonatal Mice
  • 02:53Day 2: Enzymatic Tissue Digestion and Plating of Cells
  • 05:29Day 3: Culture of Neonatal Cardiomyocytes
  • 06:01Results: Culture of Neonatal Mouse Cardiomyocytes and Representative Immunofluorescent Images
  • 07:03Conclusion

Summary

Automatic Translation

Primære mus cardiomyocyte kulturer er et af de centrale redskaber til undersøgelse af myofibrillar organisation og funktion. Følgende protokol beskriver isolering og dyrkning af primære cardiomyocytter fra neonatale mus hjerter. De resulterende cardiomyocyte kulturer kan efterfølgende anvendes til en række af biomekaniske, biokemiske og cellebaserede biologiske assays.

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