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Immunology and Infection
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体外
红血细胞溶血含量的生物大分子药物的细胞内交货的评价的pH响应Endosomolytic代理
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Ex Vivo
Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
Please note that all translations are automatically generated.
Click here for the English version.
体外
红血细胞溶血含量的生物大分子药物的细胞内交货的评价的pH响应Endosomolytic代理
DOI:
10.3791/50166-v
•
11:12 min
•
March 09, 2013
•
Brian C. Evans*
1,2
,
Christopher E. Nelson*
1,2
,
Shann S. Yu*
1,2
,
Kelsey R. Beavers
3
,
Arnold J. Kim
,
Hongmei Li
2
,
Heather M. Nelson
,
Todd D. Giorgio
2,5,6
,
Craig L. Duvall
2
1
Department of Biomedical Engineering
,
Vanderbilt University
,
2
Vanderbilt Institute for Nanoscale Science & Engineering
,
Vanderbilt University
,
3
Interdisciplinary Materials Science Program
,
Vanderbilt University
,
4
Monroe Carell Jr. Children’s Hospital
,
Vanderbilt University Medical Center
,
5
Department of Chemical & Biomolecular Engineering
,
Vanderbilt University
,
6
Department of Cancer Biology
,
Vanderbilt University
Chapters
00:01
Title
00:26
Introduction fo Endosomolysis and Hemolysis
02:16
Preparation of Buffers and Test Reagents
03:14
Blood Sample Preparation
06:05
Assay Setup and Quantification
08:39
Data Analysis
09:00
Representative Results
09:25
Discussion
Summary
Automatic Translation
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Automatic Translation
溶血试验可作为一种快速,高通量筛选,药物传递系统的细胞相容性和内货物配送endosomolytic活动的。该试验测量作为环境pH值的函数的红细胞膜的破坏。
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