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Ex Vivo røde blodceller Hemolyse Assay for Evaluering av pH-responsive Endosomolytic Agenter for cytosoliske Levering av Biomacromolecular narkotika
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
DOI:

11:12 min

March 09, 2013

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Chapters

  • 00:01Title
  • 00:26Introduction fo Endosomolysis and Hemolysis
  • 02:16Preparation of Buffers and Test Reagents
  • 03:14Blood Sample Preparation
  • 06:05Assay Setup and Quantification
  • 08:39Data Analysis
  • 09:00Representative Results
  • 09:25Discussion

Summary

Automatic Translation

En hemolyse Målingen kan brukes som en hurtig, høy-throughput skjermen stoffet leveringssystemer 'cytocompatibility og endosomolytic aktivitet for intracellulær last levering. Analysen måler avbrudd av erytrocytt membraner som en funksjon av miljømessige pH.

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