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Ex Vivo Blood Red Ensaio Hemólise celular de Avaliação de pH-responsivos agentes Endosomolytic para entrega citosólico de Drogas Biomacromolecular
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1Department of Biomedical Engineering,Vanderbilt University, 2Vanderbilt Institute for Nanoscale Science & Engineering,Vanderbilt University, 3Interdisciplinary Materials Science Program,Vanderbilt University, 4Monroe Carell Jr. Children’s Hospital,Vanderbilt University Medical Center, 5Department of Chemical & Biomolecular Engineering,Vanderbilt University, 6Department of Cancer Biology,Vanderbilt University
Chapters
- 00:01Title
- 00:26Introduction fo Endosomolysis and Hemolysis
- 02:16Preparation of Buffers and Test Reagents
- 03:14Blood Sample Preparation
- 06:05Assay Setup and Quantification
- 08:39Data Analysis
- 09:00Representative Results
- 09:25Discussion
Um ensaio de hemólise podem ser usados como um meio rápido, tela de alto rendimento de cytocompatibility sistemas de entrega de drogas e atividade endosomolytic para entrega intracelular de carga. O ensaio mede a ruptura das membranas dos eritrócitos em função do pH do meio ambiente.
