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Patient Afledt Cellekultur og isolering af CD133<sup> +</sup> Formodede kræftstamceller fra melanom
JoVE Journal
Medicine
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JoVE Journal Medicine
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
DOI:

12:16 min

March 13, 2013

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Chapters

  • 00:05Title
  • 01:38Preparing Primary Melanoma Single-cells from Tumor Tissue
  • 04:33Characterization of Primary Cell Culture and Fibroblast Depletion
  • 05:44Magnetic Cell Sorting of CD133+ and CD133- Melanoma Cells
  • 09:27Results: Magnetic Cell Sorting of CD133+ and CD133- Melanoma Cells
  • 11:38Conclusion

Summary

Automatic Translation

I denne artikel beskriver fremstillingen af ​​frisk opnåede melanomavæv i primære cellekulturer, og hvordan du fjerner forureninger af erythrocytter og fibroblaster fra tumorcellerne. Endelig beskriver vi, hvordan CD133<sup> +</sup> Putative melanom stamceller sorteres fra CD133<sup> -</sup> Hovedparten anvendelse af magnetisk aktiveret cellesortering (MACS).

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