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電気生理学基づき固体支持された膜の概要
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
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Introduction to Solid Supported Membrane Based Electrophysiology
Please note that all translations are automatically generated.
Click here for the English version.
電気生理学基づき固体支持された膜の概要
DOI:
10.3791/50230-v
•
19:56 min
•
May 11, 2013
•
Andre Bazzone
,
Wagner Steuer Costa
,
Markus Braner
,
Octavian Călinescu
,
Lina Hatahet
,
Klaus Fendler
1
Department of Biophysical Chemistry
,
Max Planck Institute of Biophysics
,
2
Buchmann Institute for Molecular Life Sciences
,
Goethe University Frankfurt
Chapters
00:01
Title
00:06
Introduction
01:25
Background: Measuring principles
02:51
Background: The SSM Setup
05:25
Experimental procedure: Mounting the cuvette
08:34
Experimental procedure: Checking membrane parameters
09:47
Experimental procedure: Adding the protein sample
10:53
Experimental procedure: Defining the flow protocol
11:48
Experimental procedure: Measuring transporter signals
14:12
Data Analysis: Reconstruction of the transporter current
15:07
Data Analysis: Representative results
17:26
Data Analysis: Artifacts & control measurements
19:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、起電膜輸送体の特性評価への応用を中心とした固体担持膜に基づいて電気生理学的手法を提案する。
Tags
Solid Supported Membrane
Electrophysiology
Membrane Protein
Proteoliposomes
Transporter
Capacitive Coupling
Transient Currents
Solution Exchange
Article
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