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JoVE Journal Medicine
Cerrahi Örnekler kaynaktan İnsan pankreas adacıklarının Lazer Diseksiyon için Geliştirilmiş Protokolü
 
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Cerrahi Örnekler kaynaktan İnsan pankreas adacıklarının Lazer Diseksiyon için Geliştirilmiş Protokolü

Article doi: 10.3791/50231
January 6th, 2013 •
1,2, 3, 1,2, 1, 2, 1,2, 2, 4, 5, 6, 7, 8, 9, 2, 10, 3, 1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

Summary
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January 6th, 2013

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Lazer mikrodisseksiyon parankiminde dakika miktarlarda seçili hücrelerin iyileşme sağlayan bir tekniktir. Burada Transkriptomik çalışmaları için kullanılacak cerrahi yöntemlerle alınan insan pankreas adacık elde etmek için bir protokol açıklar. Tüm protokol böylece toplama kolaylaştırılması insan beta hücrelerinin içsel otofloresansı, geliştirir.

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