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JoVE Journal
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脑脊液分离解剖大脑皮质外植体使用的啮齿动物胚胎
 
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脑脊液分离解剖大脑皮质外植体使用的啮齿动物胚胎

Article DOI: 10.3791/50333
March 11th, 2013

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Summary March 11th, 2013

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心室脑脊液(CSF)沐浴在早期的大脑发育在胚胎神经上皮和大脑皮质祖细胞。在这里,我们描述的方法,从不同年龄层的啮齿动物胚胎隔离室CSF,为了研究其生物学功能。此外,我们展示了我们的脑皮质外植体的解剖和文化的技术,可以为外植体生长的培养基或CSF以最小的体积。

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