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Isolering av spinalvæske fra Rodent embryo for bruk med dissekert Cerebral Kortikale explants
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1Department of Physical Medicine and Rehabilitation,VA Greater Los Angeles Healthcare System, 2Department of Pharmacology and Physiology, Institute for Neuroscience,The George Washington University School of Medicine and Health Sciences, 3Division of Genetics, Department of Medicine,Boston Children’s Hospital, 4Howard Hughes Medical Institute,Boston Children’s Hospital, 5Department of Pathology,Boston Children’s Hospital, Harvard Medical School
Chapters
- 00:05Title
- 01:24Micro-capillary Pipette and Embryo Preparations
- 02:47Ventricular CSF Collection
- 05:01Cortical Explant Dissection
- 06:51Transferring and Culturing of Cortical Explant in CSF
- 08:30CSF Collection Characteristics
- 09:20Conclusion
Den ventrikulære cerebrospinalvæsken (CSF) bader neuroepithelial og cerebral kortikale stamceller under tidlig utvikling av hjernen i embryoet. Her beskriver vi en metode utviklet for å isolere ventrikulær CSF fra gnagere embryoer i ulike aldre for å undersøke sin biologiske funksjon. I tillegg viser vi vår cerebral cortical eksplantering disseksjon og kultur teknikk som gjør det mulig for eksplantering vekst med minimale mengder kultur medium eller CSF.
