JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
开发,扩展,<em>在体内</em>从人类胚胎干细胞(hESCs)监测人类NK细胞和诱导多能干细胞(iPS细胞)
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
Development, Expansion, and
In vivo
Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
Please note that all translations are automatically generated.
Click here for the English version.
开发,扩展,<em>在体内</em>从人类胚胎干细胞(hESCs)监测人类NK细胞和诱导多能干细胞(iPS细胞)
DOI:
10.3791/50337-v
•
09:02 min
•
April 23, 2013
•
Allison M. Bock*
1,2
,
David Knorr*
1,2
,
Dan S. Kaufman
2
1
Department of Medicine (Hematology, Oncology, and Transplant)
,
University of Minnesota, Minneapolis
,
2
Stem Cell Institute
,
University of Minnesota, Minneapolis
Chapters
00:05
Title
01:18
Setting up TrypLE-passaged ES Cells into Stage I Spin Embryoid Bodies (EBs): Day of Spin EB Setup into Stage I Differentiation (Day 0)
03:16
Development, Expansion, and Characterization of NK Cells from hPSC-derived Progenitor Cells
04:19
In vivo Fluorescent and Bioluminescent Imaging to Monitor hESC-derived NK Cells and Tumor Cells in Immunodeficient Mice
06:50
Results: The Generation of Hematopoietic Progenitor Cells Using the Spin EB Approach Allows for Optimal NK Cell Development from hESCs and iPSCs
08:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本协议描述的发展,扩张和来自人类胚胎干细胞和iPS细胞的NK细胞在体内成像。
Tags
Human Embryonic Stem Cells
Induced Pluripotent Stem Cells
Natural Killer Cells
Feeder-free Differentiation
Artificial Antigen Presenting Cells
Cell Expansion
Mature Phenotype
Genetic Modification
Firefly Luciferase
In Vivo Monitoring
Dual-imaging
Adoptive Cell Therapy
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
海藻酸钠微胶囊作为一个人类胚胎干细胞(胚胎干细胞)的繁殖和分化的3D平台,以不同的谱系
在肌肉再生的老鼠模型中移植诱导多能干细胞衍生的中生代细胞样肌原体
订货单细胞和单胚胎的三维约束:高内涵筛选新设备
利用 CRISPR-Cas9 系统高效生成和编辑人胰细胞无饲养干细胞
使用非整合方法分离成人人体皮肤纤维细胞与诱导多能干细胞的生成
人类多能干细胞和免疫损伤的成熟小脑有机体可伸缩生成
在化学定义条件下,成熟肾细胞与人类诱导多能干细胞的引导分化
人类诱导多能干细胞衍生心肌细胞中的单细胞光学作用潜在测量
维持静止人类造血干细胞的文化方法
体外
人体牙浆干细胞向胰腺血统的诱导
Read Article