JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
開発、展開、および<em生体内で></em>ヒト胚性幹細胞(ヒトES細胞)からヒトNK細胞のモニタリングとや人工多能性幹細胞(性IPSC)
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
Development, Expansion, and
In vivo
Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
Please note that all translations are automatically generated.
Click here for the English version.
開発、展開、および<em生体内で></em>ヒト胚性幹細胞(ヒトES細胞)からヒトNK細胞のモニタリングとや人工多能性幹細胞(性IPSC)
DOI:
10.3791/50337-v
•
09:02 min
•
April 23, 2013
•
Allison M. Bock*
1,2
,
David Knorr*
1,2
,
Dan S. Kaufman
2
1
Department of Medicine (Hematology, Oncology, and Transplant)
,
University of Minnesota, Minneapolis
,
2
Stem Cell Institute
,
University of Minnesota, Minneapolis
Chapters
00:05
Title
01:18
Setting up TrypLE-passaged ES Cells into Stage I Spin Embryoid Bodies (EBs): Day of Spin EB Setup into Stage I Differentiation (Day 0)
03:16
Development, Expansion, and Characterization of NK Cells from hPSC-derived Progenitor Cells
04:19
In vivo Fluorescent and Bioluminescent Imaging to Monitor hESC-derived NK Cells and Tumor Cells in Immunodeficient Mice
06:50
Results: The Generation of Hematopoietic Progenitor Cells Using the Spin EB Approach Allows for Optimal NK Cell Development from hESCs and iPSCs
08:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このプロトコルは、開発、展開、およびヒトES細胞やiPS細胞から派生したNK細胞のin vivoイメージングを説明しています。
Tags
Human Embryonic Stem Cells
Induced Pluripotent Stem Cells
Natural Killer Cells
Feeder-free Differentiation
Artificial Antigen Presenting Cells
Cell Expansion
Mature Phenotype
Genetic Modification
Firefly Luciferase
In Vivo Monitoring
Dual-imaging
Adoptive Cell Therapy
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
異なる系統にヒト胚性幹細胞(hESCの)の伝播との差別化のための3Dプラットフォームとしてのアルギン酸マイクロカプセル
筋肉再生のマウスモデルにおける誘導多能性幹細胞由来メソアンジオ芽細胞様筋原性前駆細胞の移植
シングルセルおよび3D閉じ込めにおける単一胚を注文:ハイコンテントスクリーニングのための新しいデバイスを
効率的な生成および CRISPR Cas9 システムを用いた人間の膵臓細胞から Ips をフィーダー フリーの編集
腹部皮膚からの成人ヒト皮膚線維芽細胞の分離と非積分法を用いた人工多能性幹細胞の生成
ヒト多能性幹細胞からの成熟小脳オルガノイドのスケーラブルな生成と免疫染色による特徴付け
化学的に定義された条件下でのヒト人工多能性幹細胞からの成熟した腎臓ポドサイトの誘導分化
ヒト人工多能性幹細胞由来心筋細胞における単細胞光学作用電位測定
静止ヒト造血幹細胞を維持する培養法
インビトロ
膵臓系統に向けたヒト歯髄幹細胞の誘導
Read Article