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Desarrollo, la expansión y<em> En vivo</em> Monitoreo de las células NK humanas a partir de células madre embrionarias humanas (hESCs) y y células madre pluripotentes inducidas (iPSCs)
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
DOI:

09:02 min

April 23, 2013

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Chapters

  • 00:05Title
  • 01:18Setting up TrypLE-passaged ES Cells into Stage I Spin Embryoid Bodies (EBs): Day of Spin EB Setup into Stage I Differentiation (Day 0)
  • 03:16Development, Expansion, and Characterization of NK Cells from hPSC-derived Progenitor Cells
  • 04:19In vivo Fluorescent and Bioluminescent Imaging to Monitor hESC-derived NK Cells and Tumor Cells in Immunodeficient Mice
  • 06:50Results: The Generation of Hematopoietic Progenitor Cells Using the Spin EB Approach Allows for Optimal NK Cell Development from hESCs and iPSCs
  • 08:43Conclusion

Summary

Automatic Translation

Este protocolo describe el desarrollo, la expansión, y en la formación de imágenes in vivo de células NK derivadas de hESCs y iPSCs.

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