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Chapters
- 00:05Title
- 01:53Selection of BAC Clone and Region to Target
- 03:04Insert the Homology Arms into P[acman]-KO 1.0
- 04:40Recombining the Genomic Region of Interest into P[acman]-KO 1.0
- 08:59Replacing the Genomic Region with the Targeting Cassette
- 12:30Anticipated Yields
- 13:42Conclusion
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
