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Recombineering homolog rekombination konstruktioner i<em> Drosophila</em
JoVE Journal
Biology
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JoVE Journal Biology
Recombineering Homologous Recombination Constructs in Drosophila
DOI:

14:23 min

July 13, 2013

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Chapters

  • 00:05Title
  • 01:53Selection of BAC Clone and Region to Target
  • 03:04Insert the Homology Arms into P[acman]-KO 1.0
  • 04:40Recombining the Genomic Region of Interest into P[acman]-KO 1.0
  • 08:59Replacing the Genomic Region with the Targeting Cassette
  • 12:30Anticipated Yields
  • 13:42Conclusion

Summary

Automatic Translation

Homolog rekombinationsteknikker høj grad fremme<em> Drosophila</em> Genetik ved at muliggøre skabelsen af ​​molekylært præcise mutationer. Den nylige vedtagelse af recombineering tillader en at manipulere store stykker af DNA og omdanne dem til<em> Drosophila</em<sup> 6</sup>. De metoder, der præsenteres her kombinere disse teknikker til hurtigt at frembringe store homologe rekombinationsvektorer.

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