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Montering af nukleosomal Arrays fra rekombinant Core Histoner og nukleosom Positioning DNA
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JoVE Journal Biology
Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Montering af nukleosomal Arrays fra rekombinant Core Histoner og nukleosom Positioning DNA

DOI:
10.3791/50354-v

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10:40 min

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September 10, 2013

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, , , , ,
1Biochemistry and Molecular Biology,Colorado State University

Chapters

  • 00:05Title
  • 01:37Assembly of Recombinant Core Histones into Octamers
  • 03:14Purification of Histone Octamers by Size Exclusion Chromatography |
  • 05:32Sedimentation Velocity Analysis of Reconstituted Nucleosomal Arrays
  • 07:50Results: Nucleosomal Arrays from Recombinant Xenopus Core Histones
  • 09:56Conclusion

Summary

Automatic Translation

Automatic Translation

En metode præsenteres for rekonstituering af model nukleosomal arrays fra rekombinante kerne histoner og tandemgentaget DNA nukleosom positionering. Vi beskriver også, hvordan sedimentationshastighed eksperimenter i den analytiske ultracentrifuge og atomic force mikroskopi (AFM) anvendes til at overvåge omfanget af nukleosomal matrix mætning efter rekonstitution.

Tags

Nucleosomal ArrayRecombinant Core HistonesNucleosome Positioning DNASedimentation VelocityAnalytical UltracentrifugeAtomic Force MicroscopyChromatin StructureChromatin Function

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