Journal
/
/
Koblede Analyser for Monitoring Proteingenfoldningstrin i<em> Saccharomyces cerevisiae</em
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
DOI:

13:52 min

July 09, 2013

,

Chapters

  • 00:05Title
  • 01:46Construction of Strains Containing FFL-GFP Plasmid
  • 02:23Preparations Prior to FFL-GFP Induction and Enzymatic Recovery Assay
  • 03:48Induction of FFL-GFP
  • 06:22Enzymatic FFL-GFP Recovery Assay
  • 07:35Fluorescence Microscopy
  • 08:55Single Cell Microscopy
  • 10:47Results: Hsp104 is Required for Efficient Refolding of Heat-denatured FFL-GFP
  • 13:36Conclusion

Summary

Automatic Translation

Denne artikel beskriver anvendelsen af ​​en ildflue luciferase-GFP-fusionsproteinet til at undersøge<em> In vivo</em> Proteinfoldning i<em> Saccharomyces cerevisiae</em>. Brug af denne reagens refoldning af en model varme-denaturerede protein kan monitoreres samtidigt ved fluorescensmikroskopi og en enzymatisk assay til at undersøge roller proteostasis netværkskomponenter i protein kvalitetskontrol.

Related Videos

Read Article