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Hele Cell Patch Clamp for Undersøgelse af mekanismerne i Infrarød Neural Stimulation
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
DOI:

08:58 min

July 31, 2013

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Chapters

  • 00:05Title
  • 01:24Preparation for Patch Clamp Recordings
  • 03:12Patch Clamp Recordings for Investigation of INS
  • 04:58Making Whole Cell Recordings
  • 06:46INS Experiments
  • 07:32Spiral Ganglion Neuron Responses to INS
  • 08:21Conclusion

Summary

Automatic Translation

Infrarød nerve stimulation er blevet foreslået som et alternativ til elektrisk stimulering i en række nerve typer, herunder dem der er forbundet med det auditive system. Denne protokol beskriver en patch-clamp-metode til at studere den mekanisme af infrarøde nervestimulation i en kultur af primære auditive neuroner.

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