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RNAi-medieret Double Gene Knockdown og gustatoriske Perception Måling i Honey Bees (<em> Apis mellifera</em>)
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Neuroscience
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JoVE Journal Neuroscience
RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

RNAi-medieret Double Gene Knockdown og gustatoriske Perception Måling i Honey Bees (<em> Apis mellifera</em>)

DOI:
10.3791/50446-v

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10:57 min

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July 25, 2013

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, ,
1School of Life Sciences,Arizona State University , 2Department of Chemistry, Biotechnology, and Food Science,Norwegian University of Life Sciences

Chapters

  • 00:05Title
  • 02:40RNAi-mediated Double Gene Knockdown
  • 05:54Proboscis Extension Response (PER) Assay
  • 07:36Results: Double Kockdown of Vitellogenin (vg) and Ultraspiracle (usp) Genes affects Gustatory Perception
  • 09:51Conclusion

Summary

Automatic Translation

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I denne protokol, beskriver vi to strategier, der samtidig undertrykker to gener (dobbelt gen knockdown) i honningbier. Så præsenterer vi, hvordan du bruger snabel forlængelse respons (PER) assay for at undersøge effekten af ​​dobbelt gen knockdown på honningbi gustatory perception.

Tags

RNAiDouble Gene KnockdownGustatory PerceptionProboscis Extension Response (PER) AssayHoney BeesApis MelliferaVitellogenin (vg)Ultraspiracle (usp)Gene ExpressionBehavioral MaturationForagingLearning And Memory

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