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Proteomiske Prøveforberedelse fra formalin Faste og Paraffin Embedded Tissue
JoVE Journal
Biology
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JoVE Journal Biology
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Proteomiske Prøveforberedelse fra formalin Faste og Paraffin Embedded Tissue

DOI:
10.3791/50589-v

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09:20 min

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September 02, 2013

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1Department of Proteomics and Signal Transduction,Max Planck Institute of Biochemistry

Chapters

  • 00:05Title
  • 01:46Preparation of Tissue Slices for Microdissection and Sample Lysis
  • 03:20Sample Processing
  • 04:51Quantification of the Peptides
  • 05:38Fractionation of the Lys-C and Tryptic Peptides
  • 07:29Results: Peptide Quantification
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

Archival formalin faste og paraffinindlejret (FFPE) kliniske prøver er værdifuldt materiale til undersøgelse af sygdomme. Her demonstrerer vi en prøveforberedelse workflow tillader indgående proteomisk analyse af mikrodissekeres FFPE væv.

Tags

Keywords: ProteomicSample PreparationFASPLysCTrypsinPeptide FractionationQuantitative AnalysisBiomarkersDrug Targets

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