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修正了福尔马林蛋白质组学样品的制备和石蜡包埋组织
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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
Please note that all translations are automatically generated.
Click here for the English version.
修正了福尔马林蛋白质组学样品的制备和石蜡包埋组织
DOI:
10.3791/50589-v
•
09:20 min
•
September 02, 2013
•
Jacek R. Wiśniewski
1
Department of Proteomics and Signal Transduction
,
Max Planck Institute of Biochemistry
Chapters
00:05
Title
01:46
Preparation of Tissue Slices for Microdissection and Sample Lysis
03:20
Sample Processing
04:51
Quantification of the Peptides
05:38
Fractionation of the Lys-C and Tryptic Peptides
07:29
Results: Peptide Quantification
08:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
档案福尔马林固定,石蜡包埋(FFPE)的临床样本进行疾病调查有价值的材料。在这里,我们展示了一个样品制备工作流程允许显微FFPE组织的深入蛋白质组学分析。
Tags
Proteomic
Sample Preparation
FASP
LysC
Trypsin
Peptide Fractionation
Quantitative Analysis
Biomarkers
Drug Targets
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