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由外显子捕获和大规模平行测序检测体细胞遗传学改变在肿瘤标本
 

由外显子捕获和大规模平行测序检测体细胞遗传学改变在肿瘤标本

Article doi: 10.3791/50710
October 18th, 2013

Summary October 18th, 2013

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我们描述条形码DNA文库和随后的杂交为基础的外显子捕获检测的关键癌症相关的基因突变在临床肿瘤标本通过大规模并行的“下一代”测序的准备。靶向外显子的测序提供了高吞吐量,低成本,以及深的序列覆盖率的好处,从而产生较高的灵敏度,用于检测低频率突变。

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