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线性扩增介导的PCR - 未知侧翼DNA的遗传物质和表征本地化
JoVE Journal
Biology
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JoVE Journal Biology
Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
DOI:

11:58 min

June 25, 2014

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Chapters

  • 00:05Title
  • 01:33Preamplifying the Vector Genome Junctions
  • 03:19Magnetic Separation of the PCR Products
  • 04:39LAM PCR Preparation
  • 07:39Non-restrictive LAM PCR Preparation
  • 08:38Exponential Amplifications and Product Clean-Up
  • 10:41Electrophoresis Analysis and Vector Insertion Site Analysis
  • 11:26Conclusion

Summary

Automatic Translation

线性扩增介导的(LAM)-PCR方法是开发,以确定基因组中的整合型病毒载体的精确位置的方法。该技术已经发展成为学习中的基因治疗的患者中,新颖的矢量技术,T-细胞的多样性,癌症干细胞模型等生物安全克隆动力学的优越方法

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