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Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
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Chapters
- 00:05Title
- 01:49Preparation of Hypothalamic Brain Slices
- 04:29Patch-clamp Recording
- 06:32Measurement of Intracellular Ca2+
- 07:38Results: Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
- 09:05Conclusion
Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.
