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Salvataggio di virus ricombinante malattia di Newcastle da cDNA
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1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Global Health and Emerging Pathogens Institute,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai, 4Department of Microbiology and Immunology, School of Medicine and Dentistry,University of Rochester
Chapters
- 00:05Title
- 01:37Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
- 02:40Transfection of Mammalian Cells
- 03:48Co-culture of Mammalian Cells with Avian Cells
- 05:22Infection of Chicken Embryonated Eggs
- 06:31Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
- 07:52Hemagglutination (HA) Assay
- 09:03Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
- 10:33Conclusion
Virus della malattia di Newcastle (NDV) è stata ampiamente studiata negli ultimi anni per sviluppare nuovi vettori per la vaccinazione e terapia, tra gli altri. Questi studi sono stati possibili a causa di tecniche di salvataggio virus ricombinante da cDNA, come quelle descritte.
