JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
从救援的cDNA重组新城疫病毒
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Rescue of Recombinant Newcastle Disease Virus from cDNA
Please note that all translations are automatically generated.
Click here for the English version.
从救援的cDNA重组新城疫病毒
DOI:
10.3791/50830-v
•
10:55 min
•
October 11, 2013
•
Juan Ayllon
2
,
Adolfo García-Sastre
2,3
,
Luis Martínez-Sobrido
1
Department of Microbiology
,
Icahn School of Medicine at Mount Sinai
,
2
Global Health and Emerging Pathogens Institute
,
Icahn School of Medicine at Mount Sinai
,
3
Department of Medicine
,
Icahn School of Medicine at Mount Sinai
,
4
Department of Microbiology and Immunology, School of Medicine and Dentistry
,
University of Rochester
Chapters
00:05
Title
01:37
Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
02:40
Transfection of Mammalian Cells
03:48
Co-culture of Mammalian Cells with Avian Cells
05:22
Infection of Chicken Embryonated Eggs
06:31
Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
07:52
Hemagglutination (HA) Assay
09:03
Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
10:33
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
新城疫病毒(NDV)已被广泛研究,在过去的几年里,以开发新的载体接种疫苗和治疗,等等。这些研究已经可能由于技术由cDNA拯救重组病毒,如那些我们在这里描述。
Tags
Newcastle Disease Virus
NDV
Avulavirus
Reverse Genetics
CDNA
Recombinant Virus
Viral Genome
Gene Modification
Vector
Vaccination
Therapy
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
质粒DNA重组流感病毒的一代
从新型H7N9流感病毒利用杆状病毒表达系统的功能重组血凝素和神经氨酸酶蛋白的表达
可扩展的高通量选择从噬菌体展示合成抗体库
从排序的永生B细胞产生的重组人IgG单克隆抗体
中性粒细胞分离和分析,以确定他们的淋巴瘤细胞的敏感性对治疗药物的作用
来自新世界Zika病毒感染性克隆的重组病毒的救援和表征
重组原型泡沫病毒整合纯化过程中核酸污染的检测与去除
快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒
感染 Ifnar1 小鼠 Immunoprivileged 器官寨卡病毒特异 T 细胞反应的评价
原
发性鸡初囊-细胞培养模型研究传染性囊内病病毒发病机制
Read Article