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1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Global Health and Emerging Pathogens Institute,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai, 4Department of Microbiology and Immunology, School of Medicine and Dentistry,University of Rochester
Chapters
- 00:05Title
- 01:37Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
- 02:40Transfection of Mammalian Cells
- 03:48Co-culture of Mammalian Cells with Avian Cells
- 05:22Infection of Chicken Embryonated Eggs
- 06:31Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
- 07:52Hemagglutination (HA) Assay
- 09:03Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
- 10:33Conclusion
Вируса болезни Ньюкасла (NDV) была тщательно изучена в последние несколько лет, чтобы развить новые векторы для вакцинации и терапии, среди других. Эти исследования стало возможным благодаря методов по спасению рекомбинантный вирус из кДНК, таких, как мы описываем здесь.
